One possible reason this might happen is that the Application Pool in IIS is configured to run under some custom account and this account either doesn't exist or a wrong password has been provided, or the password has been changed. Look at the advanced properties of the Application Pool in IIS for which account it uses.
Also the Event Log might contain more information as to why the Application Pool is stopping immediately on the first request.
Rapid-Fail Protection:
[rapidfailProtection] If true, the application pool is shut down if there are a specified number of worker process crashes (Maximum Failures) within a specified time period (Failure Interval). By default, an application pool is shut down if there are 5 crashes within a 5 minute of Interval.
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